A satellite workshop was held at the Methods and Applications in Fluorescence meeting at UCSD, San Diego, August 19-20, with the aim of providing dissemination for beginner and intermediate users of FRET and discussing community efforts. On the first day, lectures provided the historical context of the discovery and mathematical description of FRET and discussed different approaches of measuring FRET on the single-molecule level and live-cell imaging. In addition, breakout sessions discussed practical aspects of performing measurements, preparing samples and the workflow of data analysis in solution-based single-molecule FRET. On the second day, various approaches for measuring dynamics in single-molecule FRET and image correlation spectroscopy from nanoseconds to seconds-minutes were presented. Application talks discussed FRET experiments in viruses, fluorescent-base analogs and the combination of FRET and super-resolution microscopy.
FRET in Biophysics at BPS 2019
During the 63rd Annual Meeting of the Biophysical Society Meeting in Baltimore, 2019, and in coordination with the Biophysical Society and the Biological Fluorescence Subgroup, the “FRET in Biophysics” discussion forum was conceived to rally the scientific community with special interest in quantitative fluorescence methods, with emphasis in Förster Resonance Energy Transfer (FRET). Expert panelists were invited to provide different perspectives on (i) quantitative FRET imaging, (ii) future opportunities for quantitative FRET, (iii) the users view of FRET, (iv) FRET for kinetic analysis, and (v) challenges in software development with focus on interoperability. The panelists highlighted the strength of fluorescence image spectroscopy to address challenging cellular biophysical questions, presented cutting edge development in single-molecule FRET, reported on the challenge in empowering non-experts to use fluorescence as an analytical technique, and the need for lab-wide documented implementation of published and tested algorithms and data exchange formats for fluorescence based integrative modeling and inter-operable software. The short presentations were preceded by polling the community into FRET challenges: activities that serve to establish recommended procedures, validate algorithms and convey the quantitative aspect of FRET in benefit of the FRET community. In the open panel discussion, the participants agreed that (i) the organized dissemination of knowledge, (ii) the recommendations, validations, and data sharing without impingement of scientific freedom, (iii) and an improved participation of the field are essential for further development of the field.
On occasion of the 100th anniversary of his birth and 65 years after his first publication on resonance energy transfer, the first international meeting on Förster resonance energy transfer in the life sciences took place in Göttingen, Germany, in 2011 to honor Prof. Theodor Förster’s achievements by celebrating FRET as one part of his rich scientific legacy at its original site. In 2016, the FRET meeting was renewed and again held in Göttingen. More information can be found here.